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One example of chromatography used in organic analysis is gas chromatography.
Gas chromatography is a technique used to separate and analyse volatile compounds in a sample. The sample is injected into a column filled with a stationary phase, and a carrier gas is used to push the sample through the column. As the sample travels through the column, it interacts with the stationary phase, causing different compounds to separate based on their physical and chemical properties. The separated compounds are then detected and identified using a detector, such as a mass spectrometer.
Another example of chromatography used in organic analysis is liquid chromatography. This technique is used to separate and analyse non-volatile compounds in a sample. The sample is injected into a column filled with a stationary phase, and a liquid solvent is used to push the sample through the column. As the sample travels through the column, it interacts with the stationary phase, causing different compounds to separate based on their physical and chemical properties. The separated compounds are then detected and identified using a detector, such as a UV-Vis spectrophotometer.
Overall, chromatography is a powerful tool in organic analysis, allowing scientists to separate and identify different compounds in a sample. By using different types of chromatography, scientists can analyse a wide range of organic compounds, from volatile to non-volatile, and from small molecules to large biomolecules.
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